Hepatitis B Quantitation by PCR

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Pseudonyms:  HBV, jaundice, sexually transmitted disease, STD, bloodborne disease
TURNAROUND TIME:     10  days
RealTime polymerase chain reaction (PCR) that amplifies viral DNA. The assay is based on a highly conserved region of the viral DNA that provides for the detection of all common HBV genotypes.
  • Collect:Prefer 5ml whole blood in an EDTA   tube which will then be processed to obtain serum or plasma.
  • Min. Collection: 1.0 ml whole blood.
  • Transport: Separated serum or plasma is sent at room temperature to the main laboratory. The serum should be stored frozen within 24 hours of collection.
  • Stability: Ambient: up to 24 hours.
  • Unacceptable Conditions: Frozen or severely hemolyzed blood. Clotted blood.         
    Contact the Molecular Laboratory at 918-502-1721 to obtain further information.
Incidence:  1 in 181 in the United States. About 10% are new cases per year and 0.5% die of the condition each year.
Disease Characteristics: HBV symptoms can include flu-like symptoms, weakness and jaundice. Many adults recover over a period of months but the virus can also cause chronic hepatitis which can lead to serious complications such as cirrhosis of the liver, liver cancer, liver failure and death. Liver complications may manifest in edema, hemorrhage or encephalopathy.
Reference Values: none
Test Ranges:
Normal:  0
Present but not linearly quantified: 1 to 50 IU / cc
Quantitated affected range: 50 to 50 million IU / cc
Related Tests:   HCV quantitation by PCR
Clinical Sensitivity: 99%
Analytical Sensitivity: 99%

Indications for Testing

  • To monitor the status of a known HBV infection and the response to anti-viral therapy.
  • To monitor individuals after successful anti-viral therapy for recurrence of an active infection.
  • New onset of persistent flu, jaundice, anorexia or dark urine
  • Known exposure to hepatitis
  • Suspicion of chronic hepatitis (elevated liver enzymes)