Chromosome Analysis, Fetal Demise

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CHROMOSOME ANALYSIS, FETAL DEMISE

TURNAROUND TIME: 21 to 28 days

 
 
TESTING METHODOLOGY: Chromosome analysis of metaphase cells
 
SPECIMEN REQUIREMENTS:
  • Collect: villus tissue from the placenta in sterile 15 ml conical tube with sterile tissue culture medium or sterile saline.
  • Alternate specimens: biopsies of skin, umbilical cord or other tissues may also be submitted but do not remain viable as long as villi; must be in sterile 15 ml or 50 ml conical tube with sterile tissue culture medium or sterile saline.
  • Min. Collection: 3 x 3 mm tissue
  • Transport: specimen in sterile fluid at 20-25oC
  • Stability: Ambient: 24 hours; Refrigerated: 24 hours; Frozen: unacceptable
  • Unacceptable Conditions: Frozen or clotted specimens; specimens in non-sterile fluids and containers

A Cytogenetics Laboratory Test Requisition must accompany the specimen. Contact the Cytogenetics Laboratory at 918-502-1722 to obtain further information.

INTERPRETIVE DATA:
 
 
Methods: Giemsa band (G-band) analysis at approximately a 400 - 500 band level; 2 karyotypes, analysis of 5 metaphase cells and count of 20 metaphase cells
 
Test Variables: This study involves culturing of living cells. Therefore, turnaround times given represent average times which are subject to multiple variables.
 
Indications for Use:
  • To identify constitutional chromosome abnormalities associated with fetal demise