Fish Analysis, Acute Myeloid Leukemia (AML) Profile

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FISH ANALYSIS, ACUTE MYELOID LEUKEMIA (AML) PROFILE

Pseudonyms: Acute Myeloid Leukemia Panel, AML Panel, AML

TURNAROUND TIME: 7 to 10 days
 
 
TESTING METHODOLOGY: Fluorescence in situ hybridization
 
SPECIMEN REQUIREMENTS:
  • Collect: 2 mL bone marrow aspirate in sodium heparin (green); 5 mL peripheral blood in sodium heparin (green) also acceptable
  • Minimum Collection: 1 mL bone marrow or 2 mL peripheral blood
  • Transport: bone marrow or peripheral blood in sodium heparin (green) at 20-25°C; specimen should arrive in the laboratory within 24 hours of collection
  • Stability: Ambient: 24 hours; Refrigerated: 24 hours; Frozen: unacceptable
  • Unacceptable Conditions: Frozen or clotted specimens; paraffin-embedded specimens; specimens in anticoagulants other than sodium heparin

A Cytogenetics Laboratory Test Requisition must accompany the specimen. Contact the Cytogenetics Laboratory at 918-502-1722 to obtain further information.

INTERPRETIVE DATA:
 
Test Summary: Probes included: PML/RARA, RUNX1/RUNX1T1 (AML1/ETO), CBFB and MLL
 
 
Methods: A dual-color dual-fusion FISH analysis performed on interphase cells using a probe for the PML gene on chromosome 15q22 and a probe for the RAR-alpha gene on chromosome 17q21; analysis of 200 interphase cells.
 
A dual-color, dual-fusion FISH analysis performed on interphase cells using a probe for the AML1 (RUNX1) gene on chromosome 21q22 and a probe for the ETO (RUNX1T1) gene on chromosome 8q22; analysis of 200 interphase cells.
 
A dual-color FISH analysis performed on interphase cells using a break-apart probe for the CBFB gene of chromosome 16q22 which can detect the inv(16)(p13q22) and t(16;16); analysis of 200 interphase cells.
 
A dual-color, break-apart FISH analysis performed on interphase cells using probes for 5' and 3' ends of the MLL gene on chromosome 11q23; analysis of 200 interphase cells.
 
Interpretation: A positive result is reported when the percent of cells with an abnormality exceeds the normal reference range for the probes. The detection of an abnormal clone indicates a diagnosis of AML with the specific chromosome abnormality. A negative result indicates no abnormality was observed but does not rule out the presence of a neoplastic disorder.
 
Reference Values: An interpretive report is provided for each probe set.
 
FDA Approval: This test is not approved by the FDA and it should used as an adjunct to other clinical and pathological information.
 
Indications for Use:
  • Identify the 15;17 translocation, 8;21 translocation, chromosome 16 inversion, 16;16 translocation or 11q23 translocation in patients with suspected AML.
  • Aid in determining disease prognosis and treatment options.
ADDITIONAL RESOURCES: